EFEKTIVITAS PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus Linn) PADA MENCIT (Mus musculus) INFERTIL TERHADAP KADAR LUTEINIZING HORMONE (LH) DAN JUMLAH CORPUS LUTEUM (CL)

Yanita Mutiaraning Viastika(1), Citopartusi Margaluna Purnama Tjahjani(2), Sari Eko Tuswati(3),


(1) Animal Husbandry in Wijayakusuma University
(2) Faculty of Animal Husbandry, Wijayakusuma University
(3) Faculty of Animal Husbandry, Wijayakusuma University
Corresponding Author

Abstract


Abstrak

Infertilitas dapat menyebabkan gangguan sekresi hormon reproduksi, salah satunya adalah Luteinizing Hormone (LH). Gangguan sekresi LH akan menyebabkan gangguan pada proses ovulasi. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas pemberian minyak buah merah (Pandanus conoideus Linn) dapat menurunkan kadar LH dan meningkatkan jumlah corpus luteum. Mencit dibagi menjadi empat kelompok perlakuan, P0 sebagai kontrol negatif (mencit normal), P1 sebagai kontrol positif (mencit infertil), P2 dan P3 mencit infertil yang diberi minyak buah merah dengan dosis 0.05 ml dan 0.1 ml selama 14 hari. Kemudian kadar LH diukur menggunakan Elisa. Jumlah corpus luteum dihitung pada preparat histopatologi dari ovarium mencit dengan pewarnaan HE. Hasil penelitian menunjukkan penurunan kadar LH dan peningkatan jumlah CL setelah pemberian minyak buah merah (Pandanus conoideus Linn).

Kata kunci: minyak buah merah, LH, Corpus Luteum


Abstract

Infertility can cause reproductive hormone secretion, one of which is Luteinizing Hormone (LH). LH secretion disorders will cause interference on the process of ovulation. The purpose of this research was to know the effectiveness of giving red fruit oil (Pandanus conoideus Linn) can decrease the levels of LH and increase the number of corpus luteum. The mice were grouped into four groups, that are P0 as negative control (normal mice), P1 as positive control (infertile mice), P2 and P3 infertile mice respectively given a dose of 0.05 ml and 0.1 ml red fruit oil for 14 days. After treatment, blood serum LH was measured using Elisa. Number of corpus luteum were counted from HE stained histopathological preparation of the mice ovaries. Results showed decrease in serum LH and increase in CL number after the administration of red fruit (Pandanus conoideus Linn) oil.

 

Key words: red fruit oil, LH, Corpus Luteum


References


Almatsier, S. 2010. Prinsip Dasar Ilmu Gizi. PT. Gramedia Pustaka Utama. Jakarta.

Dupont, S., Krust, A., Gansmuller, A., Dierich A., Chambon, P and Mark, M. 2000. Effect of Single and Compound Knockouts of Estrogen Receptors α (ERα) and β (ERβ) on Mouse Reproductive Phenotypes. Development Journal. 127:4277–4291.

Echternkamp, S.E., Cushman, R.A., Allan, M.F., Thallman, R.M and Gregory, K.E., 2007. Effect of Ovulation Rate and Fetal Number On Fertility In Twin Producing Cattle. Journal of Animal Science. 85:3228-3238.

El-Shahat K, Monem UMA. 2011. Effect of Dietary Supplementation with Vitamin E and/or Selenium on Metabolic and Reproductive Performance of Egyptian Baladi Ewes Under Subtropical Conditions. World Applied Sciences Journal. 12(9):1492- 1499.

Febrina, E., Dolih, G dan Taofik, R. 2007. Formulasi Sediaan Emulsi Buah Merah (Pandanus conoideus Lamk) Sebagai Produk Antioksidan Alami. Penelitian Peneliti Muda. Fakultas Farmasi. Universitas Padjadjaran.

Hariadi, S.H., Wurlina, H.A., Hermadi, B., Utomo, Rimayanti, I.N., Triana dan H. Ratnani. 2011. Ilmu Kemajiran pada Ternak. Airlangga University Press. Surabaya.

Homburg, R. 2009. Androgen Circle of Polycystic Ovary Syndrome. Human Reproduction Journal. Oxford University Press. 24(7):1548– 1555.

Jakimiuk, A.J, Weitsman, S.R., Yen, H.W., Bogusiewicz, M and Magoffin, D.A. 2002. Estrogen Receptor Alpha and Beta Expression in Theca and Granulosa Cells from Women with Polycystic Ovary Syndrome. Clinical Endocrinolology Metabolic Journal. 87:5532–5538.

Katzung, G.B. 2002. Farmakologi Dasar dan Klinik. Salemba Medika. Jakarta.

Kawashima, C., Kida, Schweigert and Miyamoto. 2009. Relationship Between Plasma Betacaroten Concentration During Peripartum Period and Ovulation in the First Follicular Wave Postpartum in Dairy Cows. Anim. Repro. Sci. 111:105-111.

Lachelin, G.C. 1991. Introduction to Clinical Reproductive Endocrinology. Butterworth Heinemann Ltd. Great Britain at the University Press. Cambridge.

Lobo, R.A. 1996. Unifying Concept for Polycystic Ovary Syndrome. In: Chang RJ Polycystic Ovary Syndrome. Serono Symposia USA Inc. Massachussets. 334.

McDowell, L.R. 2000. Vitamins in Animal and Human Nutrition. Iowa State University Press.

Meza-Herreraa, C.A., Vargas-Beltrana, F., Hector, P., Vergara-Hernandeza., Macias-Cruzb, U., Avendano-Reyesb, L., Rodriguez-Martinezc, R., Arellano-Rodriguezc, G., Francisc, G and Veliz-Derasc. 2012. Betacarotene Supplementation Increases Ovulation Rate Without an Increment in LH Secretion in Cyclic Goats. Regional Universitary Unit on Arid Lands, Bermejillo, Durango. Mexico.

Morales, C., Garcia-Pardo, L., Reymundo, C., Bellido, C., Sanchez-Criado, J.E. and Gaytan, F. 2000. Different Patterns of Structural Luteolysis in the Human Corpus Luteum of Menstruation. European Society of Human Reproduction and Embryology. 15(10):2119-2128.

Partodihardjo, S. 1987. Ilmu Reproduksi Hewan. Cetakan ke 2. Mutiara Sumber Swadaya. Jakarta.

Rebar, R., Judd, H.L., Yen, S.S.C., Rakoff, J., Vandenberg, G and Naftolin, F. 1976. Characterization of the Inappropriate Gonadotropin Secretion in Polycystic Ovary Syndrome. The Journal of Clinical Investigation. 57:1320-1329.

Samsulhadi. 2002. Obesitas dan Kesehatan Reproduksi Wanita (Aplikasi Klinis Berbasis Molekuler) dalam Tjokroprawiro A, Hendromartono, Sutjahyo Ari, Tandra A. National Obesity Symposium I. 75-83.

Sebastian, S and Bulun, S.E. 2001. A Highly Complex Organization of the Regulatory Region of the Human CYP19 (Aromatase) Gene Revealed by the Human Genome Project. Clinical Endocrinology Metabolic Journal. 86:4600 – 4602.

Schweigert, F. 2006. Beta Carotene’s in. Feed Mix. 12(1). www.agriworld.nl. [18 Juni 2016].

Soderlund, D., Canto1, P., Carranza-Lira, S and Me´ndez, J.P. 2005. No Evidence of Mutations in the P450 Aromatase Gene in Patients with Polycystic Ovary Syndrome. Human Reproduction Journal. 20(4):965-969.

Soedibyo, M. 1998. Alam Sumber Kesehatan Manfaat & Kegunaan. Balai Pustaka. Jakarta.

Untari. 2008. Formulasi Selai Dari Pasta Buah Merah (Pandanus conoideus Lamk). Jurnal Agricola. Universitas Musamus Merauke. 1(1):1.

Walters, K.A., Allan, C.M and Handelsman, D.J. 2012. Rodent Models for Human Polycystic Ovary Syndrome. Biology of Reproduction of Reproduction Journal. 86(5):1–12.

Wildan, Y. 1987. Biologi Modern. Pengantar Biologi. Tarsito. Bandung.


Full Text: PDF (Bahasa Indonesia)

Article Metrics

Abstract View : 59 times
PDF (Bahasa Indonesia) Download : 35 times

Refbacks

  • There are currently no refbacks.